Current Issue : October - December Volume : 2017 Issue Number : 4 Articles : 7 Articles
The aim of the present study was to verify the anti-cancer potentials of quercetin and procyanidin derived from grape seed against (PC-3) human prostate cancer cells and (A549) human lung cancer cells. Cytotoxicity potential of test products was assessed using MTT assay. Data recorded revealed that the IC50 was cell type and concentration dependent, where quercetin was significantly effective against A549 than PC3 cells. Also, procyanidin and quercetin mix showed a significant antagonistic reactivity in both cell lines compared with the single use of test products. Also, GSE derived procyanidin showed a lower toxicity to test A549 cells and significantly decreased IC50 value to PC3 cell line (P<0.05). Regarding the cell cycle profile of treated cancer cells, data revealed that cell cycle was mainly arrested at G2-M phase post cell treatment accompanied with significant apoptotic profile in the pre G1-phase. Moreover, the pro-apoptotic and anti-apoptotic genes were monitored in quercetin and procyanidin treated cells using real-time PCR. It was noticed that pro-apoptotic genes (Bax and P53) were up-regulated significantly to the cell control accompanied with down regulation of anti-apoptotic gene (Bcl-2). Collectively, our observations demonstrated that procyanidin and quercetin have an anti-cancer potential against PC3 and A549 cells Also, synergistic potential was detected in case of using quercetin and procyanidin mix....
Objectives. In order to enhance the immunity of cancer patients to prevent relapse or to prolong survival time, umbilical cord\nblood mononuclear cells (UCMCs) were transplanted to cancer patients. Patients and Methods. UCMCs were transfused to 63\nimmunocompromised gastrointestinal cancer patients with nonmyeloablative (NMA) conditioning regimen. Results.The clinical\nstudy showed that the number of both T and B cells increased much more rapidly after transfusion of UCMCs than that of the\ncontrol group without transplantation (...
Aim. To assess the epidemiological and clinical factors that influence the prognosis of oral and oropharyngeal squamous cell\ncarcinoma (SCC). Methods. One hundred and twenty-one cases of oral and oropharyngeal SCC were selected.The survival curves\nfor each variable were estimated using the Kaplan-Meier method. The Cox regression model was applied to assess the effect of the\nvariables on survival. Results. Cancers at an advanced stage were observed in 103 patients (85.1%). Cancers on the tongue were more\nfrequent (23.1%). The survival analysis was 59.9% in one year, 40.7% in two years, and 27.8% in 5 years.There was a significant low\nsurvival rate linked to alcohol intake (...
Background: Various incisions and approaches have been developed for lateral neck dissection. The purpose of\nthis study was to compare the surgical and cosmetic outcomes of a single low transverse incision with the hockey\nstick incision for lateral neck dissection (LND) in patients with papillary thyroid carcinoma (PTC).\nMethods: We retrospectively analyzed 97 patients with PTC who underwent therapeutic LND and total thyroidectomy\nby low transverse incision (62 patients) or hockey stick incision (35 patients). We compared the operative results,\ncosmetic outcomes, objective scar measurement, and sensory disturbance between the two groups.\nResults: The number of harvested and metastatic lymph nodes, Vancouver Scar Scale scores, and sensory change were\nnot significantly different between the two groups. The mean number of harvested lymph nodes in level II was 9.82 vs.\n9.63 (P = 0.885) (transverse incision vs. hockey stick incision, respectively) and in level V was 6.36 vs. 5.63 (P = 0.597).\nHowever, subjective satisfaction with the scar and neck contour was higher in the low transverse incision group\ncompared with the hockey stick incision group. Scores for scar consciousness and sensory change were not\nsignificantly different between the two groups.\nConclusions: A single low transverse incision may provide equivalent surgical outcomes and superior cosmetic\noutcomes compared with the hockey stick incision for LND in PTC....
Background: Overexpression of PTK7 has been found in multiple cancers and has been proposed to serve as a\nprognostic marker for intrahepatic cholangiocarcinoma. Its role in esophageal cancer, however, remains to be\nclarified. We hypothesize that PTK7 positively regulates tumorigenesis of esophageal cancer.\nMethods: We examined PTK7 expression pattern in human esophageal squamous carcinoma by Oncomine\nexpression analysis and by immunohistochemistry (IHC) staining. We knocked down PTK7 in two esophageal\nsquamous cell carcinoma cell lines, TE-5, and TE-9, by siRNA, and evaluated cell proliferation, apoptosis, and\nmigration ofPTK7-defective cells. Expressions of major apoptotic regulators and effectors were also determined by\nquantitative real-time PCR in PTK7-defective cells. We further overexpressed PTK7 in the cell to evaluate its effects\non cell proliferation, apoptosis, and migration.\nResults: Both Oncomine expression and IHC analyses showed that PTK7 is overexpressed in clinical esophageal\nsquamous cell carcinoma tumors. PTK7 siRNA suppressed cell growth and promoted apoptosis of TE-5 and TE-9.\nPTK7-defective cells further displayed reduced cellular migration that was concomitant with upregulation of Ecadherin.\nConversely, overexpression of PTK7 promotes cell proliferation and invasion, while apoptosis of the PTK7-\noverexpressing cells is repressed. Notably, major apoptotic regulators, such as p53 and caspases, are significantly\nupregulated in siPTK7 cells.\nConclusions: PTK7 plays an oncogenic role in tumorigenesis and metastasis of esophageal squamous carcinoma.\nPTK7 achieves its oncogenic function in esophageal squamous cell carcinoma partially through the negative\nregulation of apoptosis....
Background: The aims of this study were to identify the clinical significances of the size of metastatic lymph node\n(mLN) and LN ratio (LNR) and to attempt to create a risk stratification for papillary thyroid carcinoma (PTC) patients.\nMethods: We investigated the 435 PTC patients who underwent radioactive iodine (RAI) ablation treatment\nfollowing thyroid surgery. The patients were classified into two groups (micrometastasis (pN1mic) � 0.2 cm and\nmacrometastasis (pN1mac) > 0.2 cm) and were stratified into the following three risk groups: group I (pN1mic,\nLNR � 0.5); group II (pN1mic, LNR > 0.5 or pN1mac, LNR � 0.5); and group III (pN1mac with LNR > 0.5). And then we\ninvestigated the association of the classified groups and variable clinicopathologic factors.\nResults: The clinical characteristics such as large tumor size, extrathyroidal extension, higher T stage, and greater\nnumber of mLN or LNR were significantly associated with pN1mac. The mean stimulated thyroglobulin levels were\nincreased with the patient risk groups (p = 0.02). The recurrence-free survivals were significantly different between\nthe stratified groups (p = 0.001).\nConclusions: The patient groups I, II, and III may be referred to as low-, intermediate-, and high-risk groups.\nClinicians should consider the possibility of recurrence, and the decisions about the application of RAI ablation\nbased on the size of mLN and the patient�s risk groups...
Thioredoxin reductase 1 (TXNRD1) which is a selenocysteine-containing protein is overexpressed in many malignancies. Its role\nin the hepatocellular carcinoma (HCC) prognosis has not been investigated. In this study, we investigated whether TXNRD1\nfunctions as an independent prognostic factor for HCC patients.We found TXNRD1 was overexpressed in HCC tissues and cells,\nimmunohistochemical analysis suggested TXNRD1 was elevated in 57 of 120 (47.5%) clinical samples, and its level was increased\nwith the increasing clinical stage. In addition, TXNRD1 expression was positively correlated with clinical stage (...
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